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The first follow-up meeting was held in October 1998 to discuss implementation of the March recommendations. Representatives from DOE and the U.K.s Medical Research Council were present to develop a coordinated strategy and share expertise in this international effort. Recommendations Recommendations for structural analysis, functional analysis, and resources include the following: Structural Analysis * Generate an additional 60,000 new markers, identified as crucial for scientists who are cloning genes. * Genotype inbred mouse strains and generate a low-resolution (5-cM) single-nucleotide polymorphism map to determine its value for mouse research. * Sequence and map 3 ends of partial cDNAs and improve methods for isolating missing and full-length cDNAs. * Generate 12 Mb of sequence for the first year and ramp up to 400 Mb within 5 years, obtaining a completed reference mouse genomic sequence by 2008. Functional Analysis * Develop standardized genome-wide mutagenesis protocols and improved tools and assays for characterizing phenotypes within new, specialized centers using the supermutagen ENU (ethyl nitrosourea, developed at Oak Ridge National Laboratory by William Russell). * Develop phenotyping protocols in ENU centers and by individual investigators. * Set up targeted mutagenesis programs to validate embryonic stem lines from different mouse strains for specialized uses. * Couple molecular genotyping with the construction of congenic mouse strains. Resources * Develop cryopreservation methods and facilities for maintaining mutant mouse sperm and ovaries, thus reducing the cost of maintaining live animals. * Build a new repository for live mouse strains. * Evaluate and expand some existing databases. * Train researchers in cryopreservation technology and animal pathology. |
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